RBM15 Knockout cell line (AC16)

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RBM15 Knockout cell line (AC16) Order

RBM15 Knockout cell line (AC16)
Catalog#
YKO-H823
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RBM15 Knockout cell line (AC16)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RBM15 Knockout cell line (AC16)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1：3-1：4
Species
Human
Gene Name
RBM15
Gene ID
64783
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM/F12+10% FBS
Accession
CVCL_D8DN(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 1 (based on transcript RBM15-201)
Validation: PCR+Sanger Sequencing
RBM15 Knockout cell line (AC16) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:AC16		 STR Info (Cell bank)
Cell Line:AC16 [Human hybrid]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 911911
D13S317 12131213
D7S820 101112.0101112.0
D16S539 11131113
VWA 16181618
TH01 789.3789.3
AMEL XXXX
TPOX 11111111
CSF1PO 91112.091112.0
D12S391 1821
FGA 2125
D2S1338 2023
D21S11 32.232.2
D18S51 1217
D8S1179 1415
D3S1358 1718
D6S1043 1417
PENTAE 77
D19S433 1515.2
PENTAD 39
D1S1656 1212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RBM15 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the AC16 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RBM15 gene knockout RBM15 Knockout cell line (AC16) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
RBM15 Knockout cell line (AC16)
RBM15 Knockout cell line (AC16)
Catalog#
YKO-H823
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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